Expression of integrin markers on anti- malaria effector cells in the placenta of post partum women in Western Kenya
Mwalimu, Stephen M.
Malaria in pregnancy has been linked to utero-placental vascular insufficiency and fetal fatalities.
The immunity to placental malaria may be mediated in part by monocytes and T cells that
accumulate in the placenta intervillous space. The immunological basis for retention of antimalarial
effector cells in the intervillous space and the role of leucocyte integrins in the intervillous
space is not well understood. Leucocytes use surface receptors to mediate binding on endothelial
villous walls. Among these receptors, integrins play a crucial role in leucocytes tethering and
extravasation. The surface expression of integrins and intracellular adhesion molecule-1(ICAM-
1) on T cells and monocytes in reduced incidence of placental malaria in multigravidae has not
been studied. The objective of this study was to analyse the integrins and compare their expression
patterns on peripheral and placental T cells and monocytes. Placental and peripheral leucocytes
were processed from whole blood peripheral and intervillous blood. The washed leucocytes were
stained with specific monoclonal antibodies and analysed by flow cytometry for ICAM-1, very
late leucocyte antigen-4 (VLA), leucocyte function associated antigen-1(LFA-1) and macrophage
antigen-1 (MaC-1). Microscopy was used to determine peripheral and placental malaria infections,
while parasites density was calculated from the number of malaria parasites per 300 leucocytes
and converted into parasites per microliter of blood. Statistical analysis was done using Graph Pad
Prism version 5.0. Differences in the expression of integrins between two groups and within cell
population were performed by students paired t-test. Statistical significance was considered for
probability values less than 0 .05 (˂ 0.05).The results indicated the expression pattern of most
integrins studied was altered in placental blood in presence of malaria parasites. In placental
malaria ICAM-1 and VLA-4, MaC-1 and LFA-1 expression in placental monocytes was
significantly up regulated (p
Trending Articles
More Pages to Explore .....